We present SFMetrics as a metrology toolbox for SFM, specifically aimed at biomolecules like DNA and proteins, which features (a) semi-automatic high-throughput analysis of individual molecules (b) ease of use working within MATLAB environment or as a stand-alone application (c) compatibility with MultiMode (Bruker), NanoWizard (JPK instruments), Asylum (Asylum research), ASCII, and TIFF files, that can be adjusted with minor modifications to other formats. Most existing SFM software tools are aimed at broad general use: from material-surface analysis to visualization of biomolecules. However, high-throughput analysis of SFM data is limited by the lack of versatile software tools accessible to SFM users. In ver5, the edges of the ROI box can be dragged individually.Scanning force microscopy (SFM) allows direct, rapid and high-resolution visualization of single molecular complexes irregular shapes and differences in sizes are immediately revealed by the scanning tip in three-dimensional images.
This makes it nearly impossible to draw an ROI box that runs the full width or height of the imiage. Is there any version of NanoScope Analysis where the additional digits I need can be displayed?Ģ) In NA and NS ver6, section and step height analysis region of interest boxes are drawn using the Rotating box object, which has the annoying property that it can be expanded only from its center. However, in offline analysis in version 5 Nanoscope software, the step height analysis displays height with up to 5 significant figures. The above is true for offline analysis in version 6 software and in NanoScope Analysis thru version 1.5, as far as I know. In section analysis, the height difference is based on single point values. In step height analysis, the elevation of the upper and lower levels is based on an average of a selected range of data points.
This difference in display precision is ironic, because the step height value deserves more precision, not less. In step height analysis, the same data gives a displayed step height of 200 nm, with no digits after the decimal point. In section analysis, the vertical difference between two points is reported with 3 digits after the decimal point. This message concerns features that are inferior in NanoScope Analysis when compared with a benchmark software, NanoScope ver 5.31r1.ġ) In NanoScope Analysis, Section analysis provides a high precision measurement but Step Height analysis does not.
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